Confocal Microscopy Unit

logor_irsjdThe Confocal Microscopy Unit at Hospital Sant Joan de Déu (UMCHSJD) forms part of the Daniel Bravo Center for diagnosis and research into minority diseases (CDB) and the Pediatric Institute of Rare Diseases (IPER).

It is located in the Pathological Anatomy Service, which offers a first-class record for the study of cell biology and the pathology and the pathogenesis of human maladies. Since the beginning of 2017, the main objective of the UMCHSJD has been to evaluate and improve the experimental capacities and diagnostics of human pathology within a health and academic environment. The Unit provides systems of optical microscopy, equipment for sample preparation and the maintenance and resources for subsequent imaging, processing and data. It also offers services of the so-called confocal nanoscopy, which substantially increases the resolution of the confocal microscope, allowing the visualization of individual molecules inside the cells and a resolution of up to 60 nm.

The staff at the Confocal Microscopy Unit collaborates with researchers and with clinical users in the experimental planning of confocal microscopy experiments while further facilitating advanced training in the use of microscopes and specific imaging techniques. Additionally, it provides support in the processing and analysis of the acquired datasets, designing, if desired, customized analysis routines.

The two main challenges of this Unit are, on the one hand, to supply instruments that cover the whole spectrum of applications in the field of advanced optical microscopy to researchers from the HSJD as well as to both private and public institutions, and on the other hand, to incorporate this technology, a field so new and still to be fully explored in the field of diagnosis, and make it available to the clinical specialists at the HSJD. The strategic location of the team and the close collaboration of its personnel within the Pathological Anatomy Service and Pediatric Biobank allows us to use many techniques for the preparation of samples which, along with an expert knowledge of morphology, makes the UMCHSJD a unit of quite singular characteristics.

To Address

The UMCHSJD is available to all researchers from the Research Institute, to the Sant Joan de Déu Children’s Hospital and to both public and private institutions.

Check rates, schedules and booking process.

Contact

Dra. Mònica Roldán Molina
mroldanm@sjdhospitalbarcelona.org
Tel: 93-2532145 
Servicio de Anatomia Patológica
Edificio Consultas Externas, Planta 0
Passeig Sant Joan de Déu, 2
08950 Esplugues de Llobregat (Barcelona)

Photographic gallery

A. Civera-Tregón, Dra. Janet Hoenicka & Dr.  F. Palau. Grup de Neurogenètica i Medicina Molecular. Institut de Recerca. Hospital Sant Joan de Déu. Viabilitat cel·lular de cultius primaris de neurones motores (verd) i mort cel·lular (vermell).
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A. Civera-Tregón, Dr. Janet Hoenicka & Dr. F. Palau. Group of Neurogenetics and Molecular Medicine. Institut de Recerca Sant Joan de Déu. Cell viability of motor neurons primary cultures (green) and cell death (red).
Dr. Jou, A. Codina, Dr. Roldán & Dr. R. Artuch. Mitochondrial energetic metabolism group. Institut de Recerca Sant Joan de Déu. Biobank (BHISJDI). Hospital Sant Joan de Déu. Mitochondrial mass in muscle tissue (red) and Complex IV subunit I of the mitochondrial respiratory chain (green). The corresponding pseudo-colour images show the differences intensity between the images (lower)
A. Tristan-Noguero, Dr. A. García Cazorla (Grup "Synaptic Metabolism Laboratory") & Dr. A. Consiglio (Grup "Neural Commitment and differentiation"). Institut de Recerca. Hospital Sant Joan de Déu. Dopaminergic differentiation of iPS cells
Dr. C. Jimenez-Mallebrera & M. C. Badosa. Neuromuscular Diseases Group. Human dermal fibroblasts treated with ascorbic acid for 24 hours and labeled with collagen VI. In control cells, collagen VI is located in the extracellular matrix, while in patients with mutations in the COL6A genes, secretion of collagen VI is translated into intracellular retention.




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E. Figuerola Bou & Dr. J. Mora. Developmental Tumors Group. Institut de Recerca Sant Joan de Déu. 3D study of a labeled nuclear protein distribution on primary cultures cells.
E. Prada & Dr. J. Mora. Developmental Tumors Group. Institut de Recerca. Hospital Sant Joan de Déu.
I. Gonzalez, Dr. L. Cervera, E. Bridge & Dr. Francesc Gòdia. Cellular and Bioprocess Engineering Group. DEQBA. UAB. Production of HIV virus-like particles (VPLs) in human HEK 293 cell.
Dr. Mariona Hernández-Mariné. Department of Natural Products, Plant Biology and Edaphology. Faculty of Pharmacy. UB. Biofilm formed by Cyanidium sp. Photosynthetic pigments (red) and sheath autofluorescence (green)

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Dr. M. Ramírez & Dr. LA. Ibarra. INECOL (México). Xyleborus affinis, native specie from México, is considered as a secondary vector of diseases in vegetal cultures of commercial interest.
Dr. MJ Esplandiu. Instituto de Nanociencia y Nanotecnologia. Fluorescence gradient of a pH-sensitive probe due to the formation of a light-activated proton gradient in a Si/Pt motor.
Dr. Roldán. Confocal Microscopy Unit. Pathological Anatomy Service. Institut Pediàtric de Malalties Rares. Hospital Sant Joan de Déu. Nucleus cell where the nuclear pores are observed. Picture made using the STED technique.
Dr. Roldán. Confocal Microscopy Unit. Pathological Anatomy Service. Institut Pediàtric de Malalties Rares. Sant Joan de Déu Hospital. Mitochondria in the cytoplasmatic space. Picture made using the STED technique.

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