Schedule and rates

Reservations

Reservations for all activities at the UMCHSD are made via e-mail (mroldanm@sjdhospitalbarcelona.org) and are followed by a rigorous order of reception. Members from the Research Institute and Sant Joan de Déu Children's Hospital are given priority over other users.

Booking Regulations

Schedule and rates

The Confocal Microscope Unit is open Monday to Friday, from 8:30am to 4:30pm.

Pricing quotes for 2019

Equipment

  • microscopiMultispectral Confocal Leica TCS SP8 with a high speed module and a STED 3X module for super-resolution. Laser lines: 405 nm, 488 nm and white laser for 470-670 nm excitation. Depletion lines: 592, 660 nm and 775nm. Two hybrid detectors.
  • Light microscope Leica DM5500B and DFC7000T digital color camera for the detection of light transmitted images, polarized light and fluorescence.
  • Computer workstations for fluorescence and confocal processing and analysis images (LAS X, Fiji / ImageJ, Huygens).
  • Auxiliary equipment: incubator implemented in the microscope with temperature and CO2 control, centrifuge, laminar flow chamber, freezer, CO2 incubator.

Applications

Microscopy Techniques:

  • Multichannel confocal and transmission imaging of living cells or fixed samples
  • Three-dimensional analysis and relief maps
  • Multidimensional in vivo time-lapse experiments: tracking, intracellular calcium, membrane potential, others
  • High speed Confocal Microscopy
  • Colocalization studies
  • Quantitative fluorescence studies
  • Techniques F: FRET, FRAP, photoactivation...
  • Spectrum analysis of fluorescence
  • Microscopy for multi-layered experiments and mosaics
  • Transmitted light microscopy: BF, DIC and polarized light.
  • Conventional fluorescence microscopy
  • Image processing and quantification with specialized programs: LAS X, ImageJ, Fiji
  • Nanoscopy imaging: HyVolution, STED 3X (Stimulated emission depletion 3D)

Sample Preparation:

  • Sample preparation by transmitted light and fluorescence microscopy of different types of materials (biological and non-biological)
  • Chemical bindings and cutting of biological materials
  • Immunofluorescence in cells and tissues
  • Methodologies for in vivo cell analysis and physiological studies: specific fluorochromes, ion markers (calcium), lectins and fluorescent proteins

Functions

The Confocal Microscopy Unit performs the following tasks:

  • Maintenance and coordination of use of different microscopes from the Confocal Microscope Unit.
  • Technical support in working with microscopes and image processing software for untrained users.
  • Training for self-service users
  • Advice and technical support to users
  • Completion of experimental procedures by Unit personnel

To Address

The UMCHSJD is available to all researchers from the Research Institute, to the Sant Joan de Déu Children's Hospital and to both public and private institutions.

Interesting links

Contact

Dra. Mònica Roldán Molina
mroldanm@sjdhospitalbarcelona.org
Tel: 93-2532145 
Servicio de Anatomia Patológica
Edificio Consultas Externas, Planta 0
Passeig Sant Joan de Déu, 2
08950 Esplugues de Llobregat (Barcelona)